Some online sources list the title as Twinkle, Twinkle, with a comma, but ads and playbills did not include a comma in the title.
The Best Plays of 1926-1927 lists a run of 167 performances, but The Billboard listed a run of 171 performances in its end-of-season roundup. If the production played no extra performances during the run beyond the standard eight-a-week, then 167 would be correct. But ads at the time suggested, though a bit inconclusively, that several extra holiday matinees were played so we're following The Billboard and listing 171 performances.
Different sources differently list the initials of the character played by Joe E. Brown. Some list P. T. and others list P. G. We have not been able to find a playbill or a playbill scan including a cast list for this production. We are listing P. T. but the initials may have been P. G.